PVDF BLOTTING MEMBRANE
Polyvinylidene difluoride (PVDF) membrane is a high-performance, hydrophobic membrane specifically designed for protein transfer and detection in Western blotting applications. Known for its superior protein-binding capacity, mechanical strength, and compatibility with various detection methods, PVDF membranes are widely preferred in proteomics and molecular biology labs. AXIBIO PVDF membranes deliver consistent results with high signal-to-noise ratios, making them ideal for routine as well as advanced blotting protocols.
Application
High Protein Binding Capacity
Binds >170–200 µg/cm² for efficient protein retention.
Low Background Noise
Delivers clear, sharp signal development.
Strong & Reusable Membrane
Tear-resistant and suitable for multiple reprobings.
Wide Detection Compatibility
Works with chemiluminescent, chromogenic, and fluorescent systems.
Stable & pH Resistant
Hydrophobic membrane with high signal stability, compatible across pH 2–12.
PVDF BLOTTING MEMBRANE
- High Protein Binding Capacity: >170–200 µg/cm²
- Low Background Noise: Ensures clear and sharp signal development
- Mechanical Robustness: Durable and tear-resistant; supports multiple reprobings
- Wide Detection Compatibility: Suitable for chemiluminescent, chromogenic, and fluorescent detection systems
- Hydrophobic Nature: Requires pre-wetting in methanol before use
- High Signal Stability: Minimal loss of transferred proteins during processing
- Broad pH Compatibility: Stable in a wide range of pH (2–12)
| Property | Specification |
|---|---|
| Membrane Material | Polyvinylidene difluoride (PVDF) |
| Protein Binding Capacity | ≥170–200 µg/cm² |
| Pore Size | 0.22 µm or 0.45 µm |
| Thickness | ~125–150 µm |
| Format Available | Rolls, precut sheets, and discs |
| Color | White (Opaque) |
| Solvent Resistance | Compatible with methanol, ethanol, DMSO |
| pH Stability | 2–12 |
| Storage Conditions | Room temperature; dry, clean area |
| Shelf Life | 3 years (under recommended conditions) |
| Cat No. | Size | Pore Size (µm) | No. / Pkt. |
|---|---|---|---|
| AXPVDF30020 | 300 mm x 300 mm | 0.2 μm | 10 |
| AXPVDF20020 | 200 mm x 200 mm | 0.2 μm | 10 |
| AXPVDF300X320 | 300 mm x 3 mtrs. | 0.2 μm | 1 Roll |
| AXPVDF30045 | 300 mm x 300 mm | 0.45 μm | 10 |
| AXPVDF20045 | 200 mm x 200 mm | 0.45 μm | 10 |
| AXPVDF300X345 | 300 mm x 3 mtrs. | 0.45 μm | 1 Roll |